Compagny:

Gataca Systems

Name:

Leica_CSUW1-SpinningDisk

Type:

Inverted spinning disk confocal microscope with FRAP and TIRF options. 

Microscope:

Leica DMi8

Objectives (Leica):

HC Plan Apo 10x/0.45  WD 2.8mm

HC Plan Apo 20x/0.75 WD 0.66mm

HC Plan Apo 40x/1.3oil WD 0.22mm

HC Plan Apo CS2 63x/1.4oil WD 0.14mm

HC Plan Apo 100x/1.47oil WD 0.1mm - TIRF objective

HCX IR ApoL 25x/0.95water WD 2.4mm

Optionnel Objectives (On demand):

HCX Plan Apo CS2 63x/1.3Glyc WD 0.28mm

HC Plan Apo CS2 63x/1.2water WD 0.3mm

Fluorescence ocular observation:

source for excitation: Sola Light enigme LED

Filter cubes:

DAPI - GFP - mCherry

Laser Excitation for confocal imaging:  

LASER 405/445/488/561/642

Detection:

Hamamatsu Orca flash 4  Black & White 2048 x 2048 (~ 4.2 MP); 6,5μm pixel size - Bit depth: 16 bit

Emission filters:

Pos1 : 452/45 (DAPI)

Pos2: 480/25 (CFP)

Pos3: 525/50 (GFP)

Pos4: 605/54 (RFP)

Pos5: 708/75 (farRed)

TIRF : Evolve 512 EMCCD Black & White 512x512 (~ 4.2 MP); 16μm pixel size - Bit depth: 16 bit

Filter cube :

DAPI - ex350/50-dich 400- em460/50  ( need to remove the excitation filter for TIRF) 

GFP- ex470/40-dich 495 - em525/50

TXR- ex560/40-dich585- em630/75 

Common modules

FRAP iLas2 driven by the Modular Gataca software

Gataca TIRF azimutal 

LiveSR for improvement of the resolution to 120 nm

Adaptive Focus Control

Stage Top Incubator chamber  with CO2, Temperature control

ScanSlide Metamorph

Sample Compatibility:

slide with coverglass 0.17 (#1.5)

Multiwell with coverglass or equivalent

Multi Chamber with coverglass bottom

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