Compagny:

Gataca Systems

Name:

Nikon_CSUX1-SpinningDisk

Type:

Inverted spinning disk confocal microscope with FRAP and photoablation UV355nm options. 

Microscope:

Nikon Eclipse TI2

Objectives:

Nikon S Fluor 10x/0.5  WD 1.2mm

Nikon Plan Apo LambdaD 63x/1.42oil WD 0.15mm

Nikon Plan Apo LambdaD 100x/1.45oil WD 0.13mm

Leica HC Plan Apo 100x/1.4-0.7oil WD 0.1mm - objective for photoabaltion UV355nm

Fluorescence ocular observation:

source for excitation: Leica EL6000 - metal halide

Filter cubes:

DAPI - GFP - mCherry

Laser Excitation for confocal imaging:  

Laser Diode 405 nm

laser 491 nm

Laser 561 nm

Laser 635 nm

Pulsed ps Laser 355 nm (Photoablation)

Detection:

DualCam Photometrics PRIME 95B Black & White 1200x1200px; 11μm pixel size - Bit depth: 16 bit

CAM1 Emission filters:

Pos1 : QUAD 4-band filter suited for the 4 laser lines

Pos2: 527/645 - dual bad 506-548 and 620.5-669.5

Pos3: 525/50 (GFP)

Pos4: 605/64 (RFP)

Pos5: 725/150 (farRed)

Pos6: 452/45 (DAPI)

CAM2 Emission filters (only on dual CAM mode- dichroic split at 560 nm)

Pos1 : empty  

Pos4: 609/54 

Pos5: 708/75 

Common modules

FRAP iLas2 driven by the Modular Gataca software

PhotoAblation355nm

Perfect Focus System

LiveSR for improvement of the resolution to 120 nm

Stage Top Incubator chamber  with CO2, Temperature control

Sample Compatibility:

slide with coverglass 0.17 (#1.5)

Multiwell with coverglass or equivalent

Multi Chamber with coverglass bottom

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