Compagny:

Leica Microsystems

Name:

SP8-MP

Type:

Upright confocal laser scanning microscope with multiphoton option. 

Microscope:

DM6000B

Objectives:

HC PL FLUOTAR 10x/0.4Oil  WD 0.36mm

HCX IRAPOL 25x/0.95water  WD 2.5mm

HC PLAN APO CS 40x/1.25-0.75oil WD 0.1mm

HC PLAN APO CS 63x/1.40-0.6oil WD 0.1mm

Fluorescence ocular observation:

source for excitation: Leica EL6000 - metal halide

Filter cubes:

A4 (DAPI) - exc 360/40 - em 470/40

L5 (GFP) - exc 480/40 - em 527/30

I3 exc - 470/40 - em LP515

Laser Excitation for confocal imaging:  

Laser diode 405 nm

Argon laser 458/476/488/496/514nm

DPSS Laser 561 nm

HeNe Laser 633 nm

MP laser:  Chameleon Vision II ( Femtosecond Ti:Sapphire Laser with Dispersion Precompensation) - 680 nm to 1080 nm tuning range

Detection:

3 descanned Photomultiplicator (PMT), 350 to 800 nm, adjustable spectral range 

2 descanned hybrids (HyD) 350 to 800 nm, adjustable spectral range

1 PMT Trans (transmission imaging)

for MP application:

2 non-descanned trans PMT em filter: FITC/TRITC 525/50-585/40 (MultiPhoton) or 470/22 (Second harmonic generation)

2 non-descanned HyD (direct detection close to the objective)  em filter:  FITC/TXR 525/50-617/73

Common modules

FRAP

Standard scanner from 10Hz to 1,8kHz or Resonant Scanner 12kHz

Climatic chamber (RT to 37°C) NO CO2 AVAILABLE 

Sample Compatibility:

slide with coverglass 0.17 (#1.5)

petri dishes

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