Compagny:

Leica Microsystems

Name:

SP8-X

Type:

Inverted confocal laser scanning microscope. 

Microscope:

DM6000B

Objectives:

NPLAN 5x/0.12 WD14mm

HC PLAN APO CS 10x/0.4  WD 2.2mm

HC PLAN APO CS2 20x/0.75 WD 0.62mm

HC PLAN APO CS2 40x/1.3oil WD 0.24mm

HC PLAN APO CS2 63x/1.4oil WD 0.14mm

Fluorescence ocular observation:

source for excitation: Leica EL6000 - metal halide

Filter cubes:

A4 (DAPI) - exc 340-380 - em 460/50

L5 (GFP) - exc 480/40 - em 527/30

C/Y (FRET) - exc 436/12 et 500/20 - em 467/38 et 545/46

N2.1(RFP) - exc 538/46 - LP590

Laser Excitation for confocal imaging:  

Laser diode 405 nm

Laser diode 442nm

Detection:

3 descanned Photomultiplicator (PMT), 350 to 800 nm, adjustable spectral range 

2 descanned hybrids (HyD) 350 to 800 nm, adjustable spectral range, time gating option

1 PMT Trans (transmission imaging)

Common modules

Adaptative Focus Control

Standard scanner from 10Hz to 1,8kHz or Resonant Scanner 8kHz

Navigator

OKOLAB stage Top Chamber for Temperature, CO2 and humidity control

Sample Compatibility:

slide with coverglass 0.17 (#1.5)

Multiwell with coverglass or equivalent

Multi Chamber with coverglass bottom

....