Compagny:

Leica Microsystems

Name:

SP8-UV

Type:

Inverted confocal laser scanning microscope. 

Microscope:

DMi6000B

Objectives:

HC PL FLUOTAR 10x/0.3  WD 11mm

HC PLAN APO CS2 20x/0.75 WD 0.62mm

HC PLAN APO CS2 20x/0.75IMM  WD 0.66mm

HC PLAN APO CS2 40x/1.3oil WD 0.24mm

HC PLAN APO CS2 63x/1.4oil WD 0.14mm

HCX PL APO lambda Blue CS2 63x/1.4oil WD 0.14mm

Fluorescence ocular observation:

source for excitation: Leica EL6000 - metal halide

Filter cubes:

A (DAPI) - exc 340-380 - em LP425

L5 (GFP) - exc 480/40 - em 527/30

I3 exc - 470/40 - em LP515

TXR - exc 560/40 - em 630/76

N2.1 - exc 538/46 - LP590

Laser Excitation for confocal imaging:  

Laser OPSL 355nm

Laser diode 405 nm

Argon laser 458/476/488/496/514nm

DPSS Laser 561 nm

HeNe Laser 633 nm

Detection:

2 descanned Photomultiplicator (PMT), 350 to 800 nm, adjustable spectral range 

2 descanned hybrids (HyD) 350 to 800 nm, adjustable spectral range

1 PMT Trans (transmission imaging)

Common modules

FRAP

Navigator

Live Data Mode

Adaptative Focus Control

Standard scanner from 10Hz to 1,8kHz or Resonant Scanner 8kHz

Climatic chamber (RT to 37°C) NO CO2 AVAILABLE 

Sample Compatibility:

slide with coverglass 0.17 (#1.5)

Multiwell with coverglass or equivalent

Multi Chamber with covergalss bottom

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