3D Deconvolution

3D Deconvolution 1202 views


Name:      3D Deconvolution

Speciality: Upright microscope allowing 3D observation of fixed multi-labeled samples. 

Aknowlegment/Grant for this microscope: "The authors greatly acknowledge the Nikon Imaging Centre @ Institut Curie-CNRS, member of the French National Research Infrastructure France-BioImaging (ANR10-INSB-04)”.


Detectors Camera sCMOS : Prime BSi Express (Photometrics)

Sensor Gpixel GSENSE2020BSI Scientific CMOS sensor
Active Array Size 2048 x 2048 (4.2 Megapixel)
Pixel Area 6.5
μm x 6.5μm (42.25μm2)
Sensor Area 13.3mm x 13.3mm, 18.8mm diagonal
Peak QE% >95%
Read Noise
Correlated Multi-Sampling (CMS) 1.0 e- (Median)
1.1e- (RMS)
Combined/High Gain 1.6e- (Median)
1.8e- (RMS)
Full-Well Capacity
45,000e- (Combined Gain)
10,000e- (High Gain)
1,000e- (CMS)
Dynamic Range 25,000:1 (Combined Gain)
Bit Depth
16-bit (Combined Gain)
12-bit (CMS)
11-bit (High Gain)
Readout Mode Rolling Shutter, Effective Global Shutter,
Programmable Scan Mode
Binning 2x2 (on FPGA
Microscope NI-E Upright microscope with maximum FOV of 18 mm
Options Motorized stage XYZ
Software NIS Elements AR, 2D & 3D decovolution, NIS.ai,  Clarify.ai, Denoise.ai, JOBS, GA3, Large images
Objective 10x Plan Fluor , NA : 0.30 , WD : 16,0 , Dry , Phase
20x Plan Apo VC  , NA : 0.75 , WD : 1.0 , Dry , DIC
40x Plan Fluor , NA : 1.30 , oil , DIC
60x Plan Apo VC , NA : 1.40 , WD : 0.13 , oil , DIC
100x Plan Apo VC , NA : 1.40 , WD : 0.13 , oil , DIC
Filter DAPI   377/50   409   470/40
GFP   472/30   495   520/35
Cy3   535/40   565   590/40
mcherry   562/40   593   641/75
CY5   628/40   660   692/40

Data transfert:

Connect to the isiserver.net :Login by entering : curie.net\your Curie's login and Password

How to connect to the shared file on the microscope's computer (only in the Burg building):
On your own PC:
- You have to right click on "PC" and "Map a network drive" : 
Enter the network address of the microscope to access to the users folder:  \\3Ddec-NIC\users 
- Select both options : "Reconnect at logon" and "Connect using different credentials"

- Type the username "invite" and the usual password of the facility. 

NIE-Comment par Matthieu Cortes
Institut Curie