3D Deconvolution
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DESCRIPTION
Name: 3D Deconvolution
Speciality: Upright microscope allowing 3D observation of fixed multi-labeled samples.
Aknowlegment/Grant for this microscope: "The authors greatly acknowledge the Nikon Imaging Centre @ Institut Curie-CNRS, member of the French National Research Infrastructure France-BioImaging (ANR10-INSB-04)”.
Equipments:
| Detectors | Camera sCMOS : Prime BSi Express (Photometrics) Sensor Gpixel GSENSE2020BSI Scientific CMOS sensor Active Array Size 2048 x 2048 (4.2 Megapixel) Pixel Area 6.5μm x 6.5μm (42.25μm2) Sensor Area 13.3mm x 13.3mm, 18.8mm diagonal Peak QE% >95% Read Noise Correlated Multi-Sampling (CMS) 1.0 e- (Median) 1.1e- (RMS) Combined/High Gain 1.6e- (Median) 1.8e- (RMS) Full-Well Capacity 45,000e- (Combined Gain) 10,000e- (High Gain) 1,000e- (CMS) Dynamic Range 25,000:1 (Combined Gain) Bit Depth 16-bit (Combined Gain) 12-bit (CMS) 11-bit (High Gain) Readout Mode Rolling Shutter, Effective Global Shutter, Programmable Scan Mode Binning 2x2 (on FPGA |
| Microscope | NI-E Upright microscope with maximum FOV of 18 mm |
| Options | Motorized stage XYZ |
| Software | NIS Elements AR, 2D & 3D decovolution, NIS.ai, Clarify.ai, Denoise.ai, JOBS, GA3, Large images |
| Objective | 10x Plan Fluor , NA : 0.30 , WD : 16,0 , Dry , Phase 20x Plan Apo VC , NA : 0.75 , WD : 1.0 , Dry , DIC 40x Plan Fluor , NA : 1.30 , oil , DIC 60x Plan Apo VC , NA : 1.40 , WD : 0.13 , oil , DIC 100x Plan Apo VC , NA : 1.40 , WD : 0.13 , oil , DIC |
| Filter | DAPI 377/50 409 470/40 GFP 472/30 495 520/35 Cy3 535/40 565 590/40 mcherry 562/40 593 641/75 CY5 628/40 660 692/40 |
Data transfert:
Connect to the isiserver.net :Login by entering : curie.net\your Curie's login and Password
How to connect to the shared file on the microscope's computer (only in the Burg building):
On your own PC:
- You have to right click on "PC" and "Map a network drive" :
- Enter the network address of the microscope to access to the users folder: \\3Ddec-NIC\users
- Select both options : "Reconnect at logon" and "Connect using different credentials"
- Type the username "invite" and the usual password of the facility.